This test is indicated for patients with: elevated lactic acid levels, developmental delay, vomiting, muscle weakness and fatigue, abdominal pain and difficulty in breathing.
Lactate dehydrogenase and the coenzyme NADH are used to catalyze the conversion of pyruvate to lactate. In the presence of excess NADH all pyruvate will be converted to lactate and the reduction of NADH to NAD is directly proportionate to the amount of pyruvate present in the original sample. The relative absorbance is quantified by spectrophotometry.
Detection and Reference Range
Specificity of lactate dehydrogenase has possible interference by various alpha and beta keto and hydroxyl acids. Some compounds such as malate, glyoxylate, alpha-ketobutyrate and alpha- and beta-hydroxypyruvate and phenylpyruvate act as a substrate for lactate to various degrees.
Whole blood (0.04-0.13 mmol/L)
Type: Whole Blood
Specimen Requirements:Minimum 1ml- See special instructions. Less than 1 ml will result in unsuitable pH.
Specimen Collection and Shipping: Ship frozen sample on dry ice with overnight delivery.
1) Collect sample into a chilled sodium heparin or EDTA vacutainer/tube. Fresh whole blood sample is stable for only 30 minutes.
2) Immediately after blood draw add exactly 1ml whole blood to 2ml of 7 or 8% perchloric acid in an uncoated tube (Perchloric acid kits may be obtained from EGL through client services. See Supply Request Form under Forms tab of this website.)
3) Let the sample stand for another 10 minutes in an ice bath.
4) Centrifuge at 1500 x g in a refrigerated centrifuge for 10 minutes.
5) Transfer the supernatant into a new clear uncoated tube, if supernatant is not clear, repeat centrifugation for another 10 minutes.
6) Transfer clear supernatant into a new uncoated transport tube with patient identifiers, cap securely, and keep frozen.
- Urine organic acids (OA)
- Amino Acids (AA)
- Acylcarnitine (AR)
- Mitochondrial Genome Sequencing (JD)
- Mitochondrial Genome Deletions: Southern Blot (ML)
- Mitochondrial Encephalopathy: Lactic acidosis (MELAS) (QA)
- Chronic Progressive External Opthalmoplegia (QB)
- Kearns-Sayre syndrome (QB)
- Leigh Disease (QD)
- Leber Hereditary Optic Neuropathy (LHON) (QC)
- Myoclonic Epilepsy with Ragged-Red Fiber (MERFF) (QH)
- Pearson Marrow- Pancreas syndrome (QB)
- Retinitis Pigmentosa and Ataxia (NARP) (QK)