Condition Description

Cells from many different types of solid tissues may be cultured, frozen, and stored. The establishment of a cell line may be useful for future studies. This test will produce cell cultures which can then be used for additional genetic or other analyses.

Genes (0)

Indications

This test is indicated for:
  • the establishment of fetal cell lines for current of future testing.

Methodology

The tissue specimen is minced or enzymatically disaggregated, treated with collagenase, and placed in a tissue culture vessel with media to establish a fibroblast culture. The cultures are sub-cultured into 1 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks and frozen for future use.

Specimen Requirements

When sample fails to meet the acceptable criteria, please call 470.378.2200 and ask to speak with a laboratory genetic counselor (eglgc@egl-eurofins.com).
Submit only 1 of the following specimen types
Amniotic Fluid
AF

Requirements
15 ml Sterile Conical Centrifuge tube
20-30 ml fluid, discarding the first 1-2 ml. Place in 2-3 Specimen tubes. Preferred gestational age > 15 weeks.
Collection and Shipping
Must be received in a securely closed, sterile container. Ship sample at room temperature for receipt at EGL within 24 hours of collection. Do not refrigerate or freeze.
Chorionic Villi
CV

Requirements
Sterile container with EGL transport media. If EGL media is not available, other sterile culture media can be used (RPMI, Hanks Solution)
≥15-30 mg chorionic villi/placental tissue using sterile technique. Place in sterile tube(s) with transport media. If ordering multiple tests, 30 mg is recommended. Preferred gestational age > 10-12 weeks.
Collection and Shipping
Must be received in a securely closed, sterile container. Ship sample at room temperature for receipt at EGL within 24 hours of collection. Do not refrigerate or freeze.

Special Instructions

Please call 470-378-2200 before ordering this test and ask to speak to a laboratory genetic counselor.
  • Maintain An Established Prenatal Culture (CV or AF)
  • Tissue Culture Only
  • Maintain An Established Culture (Not Prenatal)

How to Order

Requisition Forms