Condition Description

Cells from many different types of solid tissues may be cultured, frozen, and stored. The establishment of a cell line may be useful for future studies.  This test will produce cell cultures which can then be used for additional genetic or other analyses.

Genes (0)

Indications

This test is indicated for the establishment of cell lines for current or future testing.

Methodology

The tissue specimen is minced or enzymatically disaggregated, treated with collagenase, and placed in a tissue culture vessel with media to establish a fibroblast culture. The cultures are sub-cultured into 1 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks and frozen for future use.

Specimen Requirements

Listed below are EGL's preferred sample criteria. For any questions, please call 470.378.2200 and ask to speak with a laboratory genetic counselor (eglgc@egl-eurofins.com).
Submit only 1 of the following specimen types
Tissue Biopsy
TB

Requirements
Sterile Container
1-2 mm in length or > 100 mg is acceptable.
Flash freese sample upon collection using liquid nitrogen. If storage is required, store sample at -80°C or colder.
Collection and Shipping
Ship frozen sample on dry ice with overnight delivery. Not accepted on Saturday.
Skin Biopsy
SK

Requirements
Sterile container with EGL transport media or other sterile culture media (RPMI, Hanks Solution)
Obtain 1-2 cm pieces of tissue from a central location rather than a distal location to enhance cell viability and growth. Place in sterile container with EGL transport media or other sterile culture media. In the absence of media, place in a sterile container with a small amount of sterile saline. Use sterile dissection (no prep) for internal tissue.
Tissue fixed in formalin cannot be used.
Collection and Shipping
Ship sample at room temperature for receipt at EGL within 24 hours of collection. Do not refrigerate or freeze.
Muscle Biopsy
MB

Requirements
Sterile Container
1-2 mm in length or > 100 mg is acceptable.
Flash freeze sample upon collection using liquid nitrogen. If storage is required, store sample at -80°C or colder.
Collection and Shipping
Ship frozen sample on dry ice with overnight delivery. Not accepted on Saturday.

How to Order

Requisition Forms